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Image Search Results
Journal: Nature Immunology
Article Title: Cross-presentation of dead cell-associated antigens shapes the neoantigenic landscape of tumor immunity
doi: 10.1038/s41590-025-02354-w
Figure Lengend Snippet: a , Schematic illustration of the two OVA fusion constructs, LA-OVA (top) and the control mutLA-OVA (bottom). b , Western blot analysis of lysates from transfected and untransfected HeLa cells probed with anti-His (top), anti-OVA (middle) and anti-β-actin (bottom) mAbs. c , Confocal analysis of HeLa cells expressing LA-OVA (left figure, top panels) or mutLA-OVA (left figure, bottom panels). Intensity profiles for OVA (AF647) and phalloidin (AF488) channels across 10 μm (white line indicated in the confocal images) are shown on the right. Scale bars shown in confocal images (left) correspond to a size of 5μm. d - e , Flow cytometry analysis of untransfected and transfected HeLa cells before (live) and after UV treatment (UV). Overlay histograms represent intracellular staining for OVA ( d , top panels) and e ). RFP fluorescence is shown for LA-OVA and mutLA-OVA cells ( d , bottom panels). Numbers in the histogram overlay represent mean fluorescence intensity. Data are representative of 3 independent experiments.
Article Snippet: HRP-conjugated goat-anti-rabbit and
Techniques: Construct, Control, Western Blot, Transfection, Expressing, Flow Cytometry, Staining, Fluorescence